Generation of Myostatin Gene-Edited Channel Catfish (Ictalurus punctatus) via Zygote Injection of CRISPR/Cas9 System
نویسندگان
چکیده
منابع مشابه
Channel Catfish Ictalurus punctatus
We used a two-compartment, clearance volume-based model to examine rubidium and cesium Ž . pharmacokinetics in channel catfish Ictalurus punctatus after intravascular administration. We compared the apparent volumes of distribution in the central and peripheral compartments and the intercompartmental and whole-body clearances of both metals at 20.0 8C and 27.5 8C. Biological half-times of Rb we...
متن کاملMolecular characterization and differential expression of the myostatin gene in channel catfish (Ictalurus punctatus).
Myostatin is a recently discovered gene that inhibits muscle growth. In the present study, we characterized the myostatin locus and its expression in channel catfish (Ictalurus punctatus). The genomic DNA and cDNA encoding the channel catfish myostatin were cloned and sequenced. The myostatin gene has three exons encoding a protein of 389 amino acids. Comparison of the genomic sequences with th...
متن کاملHigh-quality genome assembly of channel catfish, Ictalurus punctatus
BACKGROUND The channel catfish (Ictalurus punctatus), a species native to North America, is one of the most important commercial freshwater fish in the world, especially in the United States' aquaculture industry. Since its introduction into China in 1984, both cultivation area and yield of this species have been dramatically increased such that China is now the leading producer of channel catf...
متن کاملIdentification and Characterization of Full-Length cDNAs in Channel Catfish (Ictalurus punctatus) and Blue Catfish (Ictalurus furcatus)
BACKGROUND Genome annotation projects, gene functional studies, and phylogenetic analyses for a given organism all greatly benefit from access to a validated full-length cDNA resource. While increasingly common in model species, full-length cDNA resources in aquaculture species are scarce. METHODOLOGY AND PRINCIPAL FINDINGS Through in silico analysis of catfish (Ictalurus spp.) ESTs, a total ...
متن کاملLipopolysaccharide regulates myostatin and MyoD independently of an increase in plasma cortisol in channel catfish (Ictalurus punctatus).
The effects of lipopolysaccharide (LPS) on plasma cortisol and the expression of MyoD and myostatin (MSTN) mRNAs were evaluated in channel catfish. In addition, the effect of dexamethasone (Dex) on MyoD and MSTN mRNAs was examined. For the LPS injection experiments, juvenile channel catfish were injected intraperitoneally with 1.5 mg/kg LPS or sterile PBS. Blood was collected at 1, 3, 12, and 2...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2017
ISSN: 2045-2322
DOI: 10.1038/s41598-017-07223-7